Title: Human Mesenchymal Stem Cells Osteogenic Differentiation on Dental Barrier Membrane

Abstract: In the field of tissue engineering, there are biodegradable bone implants with biocompatible synthetic polymers that provide successful results in many areas. Dental barrier membranes are bioabsorbable polylactide (PLA) membranes designed for use in many applications of guided bone regeneration (GBR). It provides a structure designed to attract, capture and retain fibroblasts and epithelial cells while protecting the area around the tooth for the development of bone and periodontal supporting tissue. The aim of this study was to evaluate the properties of dental barrier membranes that inhibit cell migration and promote bone formation differentiation using bone marrow stem cells (BMSCs) with high differentiation and proliferation properties. As a result of the study, characterization studies and cell viability experiments of the Synthetic Barrier Membrane product were carried out, and it was observed that it had a positive effect on the adherence and viability of the BMSCs.

Keywords: Dental barrier membrane; Bone marrow mesenchymal stem cell (BMSC); Bone regeneration

Başlık: İnsan Mesenkimal Kök Hücrelerinin Dental Bariyer Membran Üzerinde Osteojenik Farklılaşması

Özet: Doku mühendisliği alanında birçok alanda başarılı sonuçlar veren biyouyumlu sentetik polimerlere sahip biyobozunur kemik implantları bulunmaktadır. Dental bariyer membranlar, kılavuzlu kemik rejenerasyonunun (KKR) birçok uygulamasında kullanım için tasarlanmış biyolojik olarak emilebilir polilaktid (PLA) membranlardır. Kemik ve periodontal destek dokusunun gelişimi için dişin etrafındaki alanı korurken fibroblastları ve epitel hücrelerini çekmek, yakalamak ve tutmak için tasarlanmış bir yapı sağlar. Bu çalışmanın amacı, yüksek farklılaşma ve çoğalma özelliklerine sahip kemik iliği kök hücreleri (BMSC'ler) kullanılarak hücre göçünü önleyen ve kemik oluşumu farklılaşmasını destekleyen dental bariyer membranlarının özelliklerini değerlendirilmesidir. Çalışma neticesinde, Sentetik Bariyer Membran ürünün karakterizasyon çalışmaları ve hücre canlılık deneyi yürütülerek, kemik iliği kök hücresinin tutunumu ve canlılığına pozitif etki ettiği gözlemlenmiştir.

Anahtar kelimeler: Dental bariyer membran; Kemik iliği kök hücresi; Kemik rejenerasyonu

• Amini AR, Laurencin CT, Nukavarapu SP. Bone tissue engineering: Recent advances and challenges. Critical Reviews Biomedical Engineering 2012; 40(5): 363–408.
• Dimitriou R, Jones E, McGonagle D, Giannoudis PV. Bone regeneration: Current concepts and future directions. BMC Medine 2011; 9: 66.
• Stevens MM. Biomaterials for bone tissue engineering. Materials Today 2008; 11(5): 18–25.
• Cortellini P, Tonetti MS. Focus on intrabony defects: Guided tissue regeneration. Periodontology 2000; 22: 104-132.
• Gundry RL, Burridge PW, Boheler KR. Pluripotent stem cell heterogeneity and the evolving role of proteomic technologies in stem cell biology. Proteomics 2011; 11(20): 3947-3961.
• Simara P, Motl JA, Kaufman DS. Pluripotent stem cells and gene therapy. Translational Research 2013; 161(4): 284-292.
• Pansky A, Roitzheim B, Tobiasch E. Differentiation potential of adult human mesenchymal stem cells. Clinical Laboratory 2007; 53(1-2): 81-84.
• Toma JG, Akhavan M, Fernandes KJ, Barnabe-Heider F, Sadikot A, Kaplan DR, Miller FD. Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nature Cell Biology 2001; 3(9): 778-784.
• Cesarz Z, Tamama K. Spheroid culture of mesenchymal stem cells. Stem Cells International 2016; 2016: 9176357.
• Cordonnier, T Sohier, J. Rosset, P. Layrolle, P. Biomimetic materials for bone tissue engineering-State of the art and future trends. Advanced Engineering Materials 2011; 13(5): B135–B150.
• Carvalho SM, Oliveira AAR, Jardim CA, Melo CBS, Gomes DA, Leite MF, Pereira MM. Characterization and induction of cementoblast cell proliferation by bioactive glass nanoparticles. Journal of Tissue Engineering and Regenerative Medicine 2012; 6(10): 813-821.
• Nyman S, Lang NR, Buser D, Bragger U. Bone regeneration adjacent to titanium dental implants using guided tissue regeneration: A report of two cases. The International Journal of Oral & Maxillilofacial Implants 1990; 5(1): 9-14.